Application of CRISPR in evolution analysis, detecting and typing, virulence and antibiotic resistance regulation in food-borne pathogens / 生物工程学报

Clustered regularly interspaced short palindromic repeats (CRISPR) and its associated protein gene system can limit the horizontal gene transfer, thereby effectively preventing the invasion of foreign gene elements such as bacteriophages. CRISPR arrays of different bacteria are diverse. Based on the differences in the CRISPR system, this review summarizes the application of CRISPR in food-borne pathogen evolution analysis, detection and typing, virulence and antibiotic resistance in recent years. We also address bacterial detection typing method developed based on the characteristics of CRISPR arrays and the association of CRISPR with virulence and drug resistance of food-borne pathogens. The shortcomings of CRISPR in evolution, detection and typing, virulence and resistance applications are analyzed. In addition, we suggest standardizing CRISPR typing methods, improving and expanding the CRISPR database of pathogenic bacteria, and further exploring the co-evolution relationship between phages and bacteria, to provide references for further exploration of CRISPR functions.

SYTO/EB dyes for detection of skin viability / 中华外科杂志

<p><b>OBJECTIVE</b>the main purpose of this study is to develop an assay for detection of skin viability in situ.</p><p><b>METHODS</b>Two nucleic acid dyes, SYTO 13 and ethidium bromide (EB), are used to assess the viability of skin tissue. The viability of cells can be determined by different colors under fluorescence microscope. In this study, the recovery of skin restored at 4 degrees C for different periods is measured by SYTO/EB dyes and a type of indirect assessment, WST-1. Then both results are compared.</p><p><b>RESULTS</b>The results measured by two methods showed that the viability of skin tissue was nearly 30% after two weeks storage. At any point of experiment, the data from two methods have not obvious variance.</p><p><b>CONCLUSION</b>The assessment of skin viability by SYTO/EB is also quick, sensitive and convenient. At the same time the viability of any type of cells can be observed in situ. So it can be considered as an available method for detection of skin viability.</p>

ADHESION OF LYMPHOCYTES AND EOSINOPHILS IN PERIPHERAL BLOOD OF ASTHMA GUINEA PIG MODEL AND LUNG MICROVASCULAR ENDOTHELIAL CELLS / 解放军医学杂志

To explore the adhesion of peripheral blood lymphocytes and eosinophils and lung microvascular endothelial cells (LMEC) in asthma. Guinea pigs were divided into asthma model group and control group; the adhesion rates of isolated peripheral lymphocytes, eosinophils and the cultured LMEC monolayers were assayed.The mRNA expressions of VCAM 1 and eotaxin in LMEC were determined by RT PCR.The DNA binding activities of NF ?B and AP 1 of LMEC were detected by electrophoretic mobility shifts assay (EMSA). Under the stimulation with serum of asthma group:①the adhesion rates of lymphocytes, activated eosinophils and LMEC were significantly elevated( P